Blar i forfatter "Kirkin, Vladimir"

Viser treff 1-2 av 2

    • ATG8 Family Proteins Act as Scaffolds for Assembly of the ULK Complex SEQUENCE REQUIREMENTS FOR LC3-INTERACTING REGION (LIR) MOTIFS 

      Alemu, Endalkachew Ashenafi; Lamark, Trond; Torgersen, Knut Martin; Birgisdottir, Åsa birna; Larsen, Kenneth Bowitz; Jain, Ashish; Olsvik, Hallvard Lauritz; Øvervatn, Aud Karin; Kirkin, Vladimir; Johansen, Terje (Journal article; Tidsskriftartikkel; Peer reviewed, 2012-10-05)
      Autophagy is a lysosome-dependent degradation system conserved among eukaryotes. The mammalian Atg1 homologues, Unc-51 like kinase (ULK) 1 and 2, are multifunctional proteins with roles in autophagy, neurite outgrowth, and vesicle transport. The mammalian ULK complex involved in autophagy consists of ULK1, ULK2, ATG13, FIP200, and ATG101. We have used pulldown and peptide array overlay assays to ...

    • Structural and functional analysis of a novel interaction motif within UFM1-activating enzyme 5 (UBA5) required for binding to ubiquitin-like proteins and ufmylation 

      Habisov, Sabrina; Huber, Jessica; Ichimura, Yoshinobu; Akutsu, Masato; Rogova, Natalia; Loehr, Frank; McEwan, David G.; Johansen, Terje; Dikic, Ivan; Doetsch, Volker; Komatsu, Masaaki; Rogov, Vladimir V; Kirkin, Vladimir (Journal article; Tidsskriftartikkel; Peer reviewed, 2016-02-29)
      The covalent conjugation of ubiquitin-fold modifier 1 (UFM1) to proteins generates a signal that regulates transcription, response to cell stress, and differentiation. Ufmylation is initiated by ubiquitin-like modifier activating enzyme 5 (UBA5), which activates and transfers UFM1 to ubiquitin-fold modifier-conjugating enzyme 1 (UFC1). The details of the interaction between UFM1 and UBA5 required ...